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Addgene

PGK-DTAG-CTERM-GFP
(Plasmid #185770)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 185770 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    PGK-DTAG-CTERM
  • Vector type
    Lentiviral
  • Selectable markers
    Puromycin

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    30°C
  • Growth Strain(s)
    NEB Stable
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    GFP
  • Species
    Other
  • Insert Size (bp)
    730
  • Mutation
    none
  • Promoter PGK
  • Tag / Fusion Protein
    • V5, dTAG (C terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site BamH1 (not destroyed)
  • 3′ cloning site EcoR1 (not destroyed)
  • 5′ sequencing primer N/A
    • (Common Sequencing Primers)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    PGK-DTAG-CTERM-GFP was a gift from Alessandra Ianari & William Sellers (Addgene plasmid # 185770 ; http://n2t.net/addgene:185770 ; RRID:Addgene_185770)

  • For your References section:

    Systematic profiling of conditional degron tag technologies for target validation studies. Bondeson DP, Mullin-Bernstein Z, Oliver S, Skipper TA, Atack TC, Bick N, Ching M, Guirguis AA, Kwon J, Langan C, Millson D, Paolella BR, Tran K, Wie SJ, Vazquez F, Tothova Z, Golub TR, Sellers WR, Ianari A. Nat Commun. 2022 Sep 20;13(1):5495. doi: 10.1038/s41467-022-33246-4. 10.1038/s41467-022-33246-4 PubMed 36127368
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