SFFV-HALO-NTERM-GFP
(Plasmid
#185762)
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PurposeDegron-GFP fusion plasmid with restriction sites BamH1 and EcoR1 around GFP for subcloning
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Depositing Labs
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Sequence Information
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 185762 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backboneSFFV-HALO-NTERM
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Vector typeLentiviral
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Selectable markersPuromycin
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature30°C
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Growth Strain(s)NEB Stable
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameGFP
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SpeciesOther
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Insert Size (bp)730
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Mutationnone
- Promoter SFFV
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Tag
/ Fusion Protein
- V5, Halo (N terminal on insert)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site BamH1 (not destroyed)
- 3′ cloning site EcoR1 (not destroyed)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
SFFV-HALO-NTERM-GFP was a gift from Alessandra Ianari & William Sellers (Addgene plasmid # 185762 ; http://n2t.net/addgene:185762 ; RRID:Addgene_185762) -
For your References section:
Systematic profiling of conditional degron tag technologies for target validation studies. Bondeson DP, Mullin-Bernstein Z, Oliver S, Skipper TA, Atack TC, Bick N, Ching M, Guirguis AA, Kwon J, Langan C, Millson D, Paolella BR, Tran K, Wie SJ, Vazquez F, Tothova Z, Golub TR, Sellers WR, Ianari A. Nat Commun. 2022 Sep 20;13(1):5495. doi: 10.1038/s41467-022-33246-4. 10.1038/s41467-022-33246-4 PubMed 36127368
