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pET28-His6-Ubc9-K14R
(Plasmid #185759)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 185759 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pET28
  • Backbone manufacturer
    Novagen
  • Backbone size w/o insert (bp) 5368
  • Total vector size (bp) 5769
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Low Copy

Gene/Insert

  • Gene/Insert name
    SUMO-conjugating enzyme UBC9
  • Alt name
    UBE2I
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    483
  • Mutation
    K14R
  • Entrez Gene
    UBE2I (a.k.a. C358B7.1, P18, UBC9)
  • Promoter T7
  • Tag / Fusion Protein
    • Thrombin-cleavable His6-tag (N terminal on backbone)

Cloning Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pET28-His6-Ubc9-K14R was a gift from Jeffrey Bode (Addgene plasmid # 185759 ; http://n2t.net/addgene:185759 ; RRID:Addgene_185759)

  • For your References section:

    Site-Specific Protein Ubiquitylation Using an Engineered, Chimeric E1 Activating Enzyme and E2 SUMO Conjugating Enzyme Ubc9. Akimoto G, Fernandes AP, Bode JW. ACS Cent Sci. 2022 Feb 23;8(2):275-281. doi: 10.1021/acscentsci.1c01490. Epub 2022 Feb 9. 10.1021/acscentsci.1c01490 PubMed 35237717
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