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pSub-MBP-GFP(2M-IKQE)
(Plasmid #185757)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 185757 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pMAL-C2
  • Backbone size w/o insert (bp) 5463
  • Total vector size (bp) 7422
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Low Copy

Gene/Insert

  • Gene/Insert name
    GFP(2M-IKQE)
  • Alt name
    sfGFP with internal LACE tag (IKQE)
  • Species
    Synthetic
  • Insert Size (bp)
    1959
  • Promoter tac
  • Tag / Fusion Protein
    • MBP (N terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site BamHI (not destroyed)
  • 3′ cloning site XhoI (not destroyed)
  • 5′ sequencing primer MBP-F
  • 3′ sequencing primer pBAD Reverse
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

GFP-I for use as a ubiquitination substrate in lysine acylation using conjugating enzymes.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pSub-MBP-GFP(2M-IKQE) was a gift from Jeffrey Bode (Addgene plasmid # 185757 ; http://n2t.net/addgene:185757 ; RRID:Addgene_185757)

  • For your References section:

    Site-Specific Protein Ubiquitylation Using an Engineered, Chimeric E1 Activating Enzyme and E2 SUMO Conjugating Enzyme Ubc9. Akimoto G, Fernandes AP, Bode JW. ACS Cent Sci. 2022 Feb 23;8(2):275-281. doi: 10.1021/acscentsci.1c01490. Epub 2022 Feb 9. 10.1021/acscentsci.1c01490 PubMed 35237717
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