pET-ChE1 v4.5
(Plasmid
#185755)
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PurposeBacterial expression of ChE1 v4.5
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Depositing Lab
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Sequence Information
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 185755 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backbonepET
- Backbone size w/o insert (bp) 5406
- Total vector size (bp) 8487
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Vector typeBacterial Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberLow Copy
Gene/Insert
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Gene/Insert nameChE1 v4.5
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Insert Size (bp)3081
- Promoter T7
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Tag
/ Fusion Protein
- His10 (C terminal on insert)
Cloning Information
- Cloning method Gibson Cloning
- 5′ sequencing primer T7
- 3′ sequencing primer T7term (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pET-ChE1 v4.5 was a gift from Jeffrey Bode (Addgene plasmid # 185755 ; http://n2t.net/addgene:185755 ; RRID:Addgene_185755) -
For your References section:
Site-Specific Protein Ubiquitylation Using an Engineered, Chimeric E1 Activating Enzyme and E2 SUMO Conjugating Enzyme Ubc9. Akimoto G, Fernandes AP, Bode JW. ACS Cent Sci. 2022 Feb 23;8(2):275-281. doi: 10.1021/acscentsci.1c01490. Epub 2022 Feb 9. 10.1021/acscentsci.1c01490 PubMed 35237717
