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Addgene

L2-AA012
(Plasmid #185751)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 185751 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pCsA
  • Backbone size w/o insert (bp) 6702
  • Total vector size (bp) 11231
  • Vector type
    Plant Expression
  • Selectable markers
    Hygromycin

Growth in Bacteria

  • Bacterial Resistance(s)
    Spectinomycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert 1

  • Gene/Insert name
    HygR2
  • Alt name
    Hygromycin resistance
  • Species
    Synthetic

Gene/Insert 2

  • Gene/Insert name
    eGFP
  • Species
    Synthetic
  • Tag / Fusion Protein
    • membrane localization tag Lti6b (C terminal on insert)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    L2-AA012 was a gift from Peter Szoevenyi (Addgene plasmid # 185751 ; http://n2t.net/addgene:185751 ; RRID:Addgene_185751)

  • For your References section:

    An Agrobacterium-mediated stable transformation technique for the hornwort model Anthoceros agrestis. Frangedakis E, Waller M, Nishiyama T, Tsukaya H, Xu X, Yue Y, Tjahjadi M, Gunadi A, Van Eck J, Li FW, Szovenyi P, Sakakibara K. New Phytol. 2021 Jun 2. doi: 10.1111/nph.17524. 10.1111/nph.17524 PubMed 34076270
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