pWS3866
(Plasmid
#185743)
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Purpose(Empty Backbone) Sub-array backbone 2/4
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 185743 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backboneColE1
- Backbone size (bp) 1961
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Vector typeYeast Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Resource Information
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Supplemental Documents
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pWS3866 was a gift from Rodrigo Ledesma-Amaro (Addgene plasmid # 185743 ; http://n2t.net/addgene:185743 ; RRID:Addgene_185743) -
For your References section:
Inducible expression of large gRNA arrays for multiplexed CRISPRai applications. Shaw WM, Studena L, Roy K, Hapeta P, McCarty NS, Graham AE, Ellis T, Ledesma-Amaro R. Nat Commun. 2022 Aug 25;13(1):4984. doi: 10.1038/s41467-022-32603-7. 10.1038/s41467-022-32603-7 PubMed 36008396