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Addgene

pTPH413
(Plasmid #185728)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 185728 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    p15a
  • Vector type
    Bacterial Expression, CRISPR

Growth in Bacteria

  • Bacterial Resistance(s)
    Chloramphenicol, 25 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Low Copy

Gene/Insert 1

  • Gene/Insert name
    ecTadA(8e)-neNme2-C
  • Insert Size (bp)
    3834
  • Mutation
    Nme2Cas9 P6S/D16A/E33G/K104T/D152A/F260L/A263T/A303S/D451V/E520A/H588A/R646S/F696V/G711R/I758V/H767Y/E932K/N1031S/R1033G/K1044R/Q1047R/V1056A

Gene/Insert 2

  • Gene/Insert name
    Nme2Cas9 sgRNA
  • Insert Size (bp)
    150

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pTPH413 was a gift from David Liu (Addgene plasmid # 185728 ; http://n2t.net/addgene:185728 ; RRID:Addgene_185728)

  • For your References section:

    High-throughput continuous evolution of compact Cas9 variants targeting single-nucleotide-pyrimidine PAMs. Huang TP, Heins ZJ, Miller SM, Wong BG, Balivada PA, Wang T, Khalil AS, Liu DR. Nat Biotechnol. 2022 Sep 8. pii: 10.1038/s41587-022-01410-2. doi: 10.1038/s41587-022-01410-2. 10.1038/s41587-022-01410-2 PubMed 36076084
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