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Addgene

pTPH405c
(Plasmid #185726)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 185726 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    ColE1
  • Vector type
    Bacterial Expression, CRISPR

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Low Copy

Gene/Insert

  • Gene/Insert name
    gIII(N)-NpuN-NpuC-gIII(C) single PAM linker
  • Insert Size (bp)
    1722

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Please note there is a single mutation in LuxA/B- E353K. Depositor confirms that LuxAB is not necessary for plasmid function.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pTPH405c was a gift from David Liu (Addgene plasmid # 185726 ; http://n2t.net/addgene:185726 ; RRID:Addgene_185726)

  • For your References section:

    High-throughput continuous evolution of compact Cas9 variants targeting single-nucleotide-pyrimidine PAMs. Huang TP, Heins ZJ, Miller SM, Wong BG, Balivada PA, Wang T, Khalil AS, Liu DR. Nat Biotechnol. 2022 Sep 8. pii: 10.1038/s41587-022-01410-2. doi: 10.1038/s41587-022-01410-2. 10.1038/s41587-022-01410-2 PubMed 36076084
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