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Addgene

FUGW-UBC-EGFP-2A-BoNT-B (LC)
(Plasmid #185722)

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Item Catalog # Description Quantity Price (USD)
Plasmid 185722 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    FUGW (Plasmid #14883)
  • Backbone size w/o insert (bp) 9930
  • Total vector size (bp) 11323
  • Vector type
    Mammalian Expression, Lentiviral

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    30°C
  • Growth Strain(s)
    NEB Stable
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    botulinum neurotoxin type B light chain
  • Alt name
    BoNT-B
  • Species
    Clostridium botulinum
  • Insert Size (bp)
    1371
  • Promoter hUbC

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site BsrGI (not destroyed)
  • 3′ cloning site EcoRI (not destroyed)
  • 5′ sequencing primer EGFP-C
  • 3′ sequencing primer WPRE-R
    • (Common Sequencing Primers)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    FUGW-UBC-EGFP-2A-BoNT-B (LC) was a gift from Jun Ding (Addgene plasmid # 185722 ; http://n2t.net/addgene:185722 ; RRID:Addgene_185722)

  • For your References section:

    Postsynaptic synucleins mediate endocannabinoid signaling. Albarran E, Sun Y, Liu Y, Raju K, Dong A, Li Y, Wang S, Sudhof TC, Ding JB. Nat Neurosci. 2023 Jun;26(6):997-1007. doi: 10.1038/s41593-023-01345-0. Epub 2023 May 29. 10.1038/s41593-023-01345-0 PubMed 37248337
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