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pAAV-hSyn-GFP-IRES-hSNCA (S129A)
(Plasmid #185718)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 185718 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pAAV-hSyn-EGFP
  • Backbone size w/o insert (bp) 4874
  • Total vector size (bp) 6359
  • Vector type
    AAV

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    30°C
  • Growth Strain(s)
    NEB Stable
  • Copy number
    Low Copy

Gene/Insert

  • Gene/Insert name
    synuclein, alpha
  • Alt name
    SNCA
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    423
  • Mutation
    Changed Ser 129 to Ala
  • GenBank ID
    NM_000345
  • Entrez Gene
    SNCA (a.k.a. NACP, PARK1, PARK4, PD1)
  • Promoter hSyn

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site SalI (not destroyed)
  • 3′ cloning site NheI (not destroyed)
  • 5′ sequencing primer EGFP-C
  • 3′ sequencing primer WPRE-R
    • (Common Sequencing Primers)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pAAV-hSyn-GFP-IRES-hSNCA (S129A) was a gift from Jun Ding (Addgene plasmid # 185718 ; http://n2t.net/addgene:185718 ; RRID:Addgene_185718)

  • For your References section:

    Postsynaptic synucleins mediate endocannabinoid signaling. Albarran E, Sun Y, Liu Y, Raju K, Dong A, Li Y, Wang S, Sudhof TC, Ding JB. Nat Neurosci. 2023 Jun;26(6):997-1007. doi: 10.1038/s41593-023-01345-0. Epub 2023 May 29. 10.1038/s41593-023-01345-0 PubMed 37248337
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