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2Bc-T MBP_BoMoC(ed)_W403A_F753A_6xH
(Plasmid #185713)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 185713 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pET His6 LIC cloning vector (2Bc-T)
  • Backbone manufacturer
    Scott Gradia
  • Backbone size w/o insert (bp) 5899
  • Total vector size (bp) 8419
  • Modifications to backbone
    Modified to include an N-terminal MBP tag
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Low Copy

Gene/Insert

  • Gene/Insert name
    B. mori truncated R2 RT
  • Alt name
    BoMoC
  • Species
    Bombyx mori
  • Insert Size (bp)
    2520
  • Mutation
    Changed W403 and F753 to A
  • Promoter T7
  • Tags / Fusion Proteins
    • MBP (N terminal on insert)
    • 6xHis (C terminal on insert)

Cloning Information

  • Cloning method Ligation Independent Cloning
  • 5′ sequencing primer T7 promoter
  • 3′ sequencing primer T7 terminator
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    2Bc-T MBP_BoMoC(ed)_W403A_F753A_6xH was a gift from Kathleen Collins (Addgene plasmid # 185713 ; http://n2t.net/addgene:185713 ; RRID:Addgene_185713)

  • For your References section:

    Separable structural requirements for cDNA synthesis, nontemplated extension, and template jumping by a non-LTR retroelement reverse transcriptase. Pimentel SC, Upton HE, Collins K. J Biol Chem. 2022 Mar;298(3):101624. doi: 10.1016/j.jbc.2022.101624. Epub 2022 Jan 21. 10.1016/j.jbc.2022.101624 PubMed 35065960
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