pMX-53BP1(1-1710) 3LA7A
(Plasmid #185707)

• Purpose: Mammalian Expression, Retroviral vector that encodes 7A and 3LA mutant of 53BP1. Prevents shieldin and RIF1 recruitment to sites of double-strand breaks and disrupts 53BP1 activity.
• Depositing Lab: Daniel Durocher
• Publication: Setiaputra et al Mol Cell. 2022 Apr 7;82(7):1359-1371.e9. doi: 10.1016/j.molcel.2022.01.025. Epub 2022 Feb 24.

Backbone

• Vector backbone: pMX
• Total vector size (bp): 10895
• Vector type: Mammalian Expression, Retroviral
• Selectable markers: Puromycin

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 30°C
• Growth Strain(s): NEB Stable
• Copy number: Unknown

Gene/Insert

• Gene/Insert name: 53BP1(1-1710) 3LA7A
• Species: H. sapiens (human)
• Insert Size (bp): 5130
• Mutation: T302A, S452A, S523A, S543A, S625A, S784A, and S892A and L175A, L517A, and L693A.
• GenBank ID: NM_005657.4 NM_005657.4
• Entrez Gene: TP53BP1 (a.k.a. 53BP1, TDRD30, p202, p53BP1)
• Promoter: SV40
• Tags / Fusion Proteins:
  • Flag tag (C terminal on insert)
  • HA tag (N terminal on insert)
  • HA tag (C terminal on insert)
  • tetracysteine tag (C terminal on insert)

Cloning Information

• Cloning method: Unknown
• 5′ sequencing primer: GCTTGGATACACGCCGC

Resource Information

• Supplemental Documents:
  • pMX-53BP1(1-1710) 3LA7A vAddgene (1).ape

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Industry MTA