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Addgene

p159-RV-syn-dio-mScarlet-T2A
(Plasmid #185700)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 185700 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    RV-Syn
  • Vector type
    Retroviral

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    NEB Stable
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    mScarlet
  • Alt name
    mScarlet
  • Species
    Synthetic
  • Insert Size (bp)
    822
  • Promoter Synapsin
  • Tag / Fusion Protein
    • mScarlet palmitoylation with T2A on 3'-end

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site PacI (not destroyed)
  • 3′ cloning site AscI (not destroyed)
  • 5′ sequencing primer CAAGAGGTGCaagatagggggg
    • (Common Sequencing Primers)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    p159-RV-syn-dio-mScarlet-T2A was a gift from Lynette Lim (Addgene plasmid # 185700 ; http://n2t.net/addgene:185700 ; RRID:Addgene_185700)
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