Raptor_1 shRNA
(Plasmid #1857)

• Depositing Lab: David Sabatini
• Publication: Sarbassov et al Science 2005 Feb 18;307(5712):1098-101.

Backbone

• Vector backbone: pLKO.1
• Backbone manufacturer: Stewart SA, RNA 2003 Apr; 9(4):493-501.
• Backbone size w/o insert (bp): 7032
• Vector type: Mammalian Expression, Lentiviral, RNAi
• Selectable markers: Puromycin

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): XL10-Gold
• Growth instructions: XL10-Gold Ultracompetent Cells from Stratagene. 37oC.
• Copy number: Unknown

Gene/Insert

• Gene/Insert name: Raptor
• Alt name: 65 Raptor_1
• Alt name: shRNA Raptor
• Species: H. sapiens (human)
• Insert Size (bp): 60
• Entrez Gene: RPTOR (a.k.a. KOG1, Mip1)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: AgeI (destroyed during cloning)
• 3′ cloning site: EcoRI (not destroyed)
• 5′ sequencing primer: LKO.1 5'

Resource Information

• Reference:
  • Addgene's pLKO.1 protocol
• Articles Citing this Plasmid:
  • 26 References

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

This plasmid is referred to as Raptor shRNA_2 in the science article. Sense: CCGGAGGGCC CTGCTACTCG CTTTTCTCGA GAAAAGCGAG TAGCAGGGCC CTTTTTTG. Antisense: AATTCAAAAA AGGGCCCTGC TACTCGCTTT TCTCGAGAAA AGCGAGTAGC AGGGCCC. For packaging, please use pCMV-dR8.2 dvpr (Addgene plasmid #8455) and pCMV-VSVG (Addgene plasmid #8454).