pMX-53BP1(1-1710) 3LA
(Plasmid
#185698)
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PurposeMammalian Expression, Retroviral vector that encodes a 3LA mutant of 53BP1 that disrupts three RIF1-binding motifs and abolishes the 53BP1-dependent recruitment of RIF1 to DNA double-strand breaks.
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 185698 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepMX
- Total vector size (bp) 10865
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Vector typeMammalian Expression, Retroviral
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Selectable markersPuromycin
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature30°C
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Growth Strain(s)NEB Stable
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Copy numberUnknown
Gene/Insert
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Gene/Insert name53BP1(1-1710) 3LA
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SpeciesH. sapiens (human)
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Insert Size (bp)5130
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MutationL175A, L517A, and L693A
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GenBank IDNM_005657.4 NM_005657.4
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Entrez GeneTP53BP1 (a.k.a. 53BP1, TDRD30, p202, p53BP1)
- Promoter SV40
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Tags
/ Fusion Proteins
- Flag tag (C terminal on insert)
- HA tag (C terminal on insert)
- tetracysteine tag (C terminal on insert)
Cloning Information
- Cloning method Unknown
- 5′ sequencing primer gcttggatacacgccgc (Common Sequencing Primers)
Resource Information
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Supplemental Documents
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
Please note plasmid contains a K1031E mutation in the 53BP1(1-1710) 3LA insert. This mutation does not affect plasmid function.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pMX-53BP1(1-1710) 3LA was a gift from Daniel Durocher (Addgene plasmid # 185698 ; http://n2t.net/addgene:185698 ; RRID:Addgene_185698) -
For your References section:
RIF1 acts in DNA repair through phosphopeptide recognition of 53BP1. Setiaputra D, Escribano-Diaz C, Reinert JK, Sadana P, Zong D, Callen E, Sifri C, Seebacher J, Nussenzweig A, Thoma NH, Durocher D. Mol Cell. 2022 Apr 7;82(7):1359-1371.e9. doi: 10.1016/j.molcel.2022.01.025. Epub 2022 Feb 24. 10.1016/j.molcel.2022.01.025 PubMed 35216668