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Addgene

p226-AAV-dlx-dio-mScarlet-T2A-SYPEGFP
(Plasmid #185696)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 185696 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pAAV-mDlx-dio
  • Backbone manufacturer
    addgene
  • Backbone size w/o insert (bp) 3963
  • Total vector size (bp) 7438
  • Modifications to backbone
    removal of EGFP and replace with double inverted lox sites and Tdtomato-T2A-SYPEGFP flanked by AscI and FseI sites
  • Vector type
    Mammalian Expression, Retroviral, Cre/Lox

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    NEB Stable
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    mScarlet, T2A, Synaptophysin-EGFP
  • Alt name
    mScarlet, SYP-EGFP
  • Species
    Synthetic
  • Insert Size (bp)
    2460
  • Promoter mDlx
  • Tag / Fusion Protein
    • mScarlet palmitoylation, Synaptophysin fused to EGFP

Cloning Information

  • Cloning method Gibson Cloning
  • 5′ sequencing primer GTGTCCACTCCCAGTTCAATTACA
  • 3′ sequencing primer cgtatccacatagcgtaaaaggag
    • (Common Sequencing Primers)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    p226-AAV-dlx-dio-mScarlet-T2A-SYPEGFP was a gift from Lynette Lim (Addgene plasmid # 185696 ; http://n2t.net/addgene:185696 ; RRID:Addgene_185696)
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