pPW3761 : CMVd3-HsIRE1(WLLI-GSGS)[359-362]-HaloTag
(Plasmid
#185683)
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PurposeLow-level transient expression of IF2 mutant HsIRE1 with a C-terminal HaloTag.
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Depositing Lab
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Sequence Information
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 185683 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backbonepCMVd3
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Vector typeMammalian Expression
Growth in Bacteria
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Bacterial Resistance(s)Kanamycin, 50 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameERN1
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Alt nameIRE1alpha(WLLI-GSGS[359-362])-HaloTag
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SpeciesH. sapiens (human), Synthetic
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Mutation(WLLI-GSGS)[359-362]
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Entrez GeneERN1 (a.k.a. IRE1, IRE1P, IRE1a, hIRE1p)
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Tag
/ Fusion Protein
- HaloTag (C terminal on insert)
Cloning Information
- Cloning method Gibson Cloning
Resource Information
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Supplemental Documents
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pPW3761 : CMVd3-HsIRE1(WLLI-GSGS)[359-362]-HaloTag was a gift from Peter Walter (Addgene plasmid # 185683 ; http://n2t.net/addgene:185683 ; RRID:Addgene_185683) -
For your References section:
Endoplasmic reticulum stress activates human IRE1alpha through reversible assembly of inactive dimers into small oligomers. Belyy V, Zuazo-Gaztelu I, Alamban A, Ashkenazi A, Walter P. Elife. 2022 Jun 22;11:e74342. doi: 10.7554/eLife.74342. 10.7554/eLife.74342 PubMed 35730415
