pPW3755 : HDR-HsIRE1a-10xGS-HaloTag
(Plasmid #185677)

• Purpose: HDR template used for endogenous CRISPR editing of ERN1 gene, inserting a C-terminal HaloTag.
• Depositing Lab: Peter Walter
• Publication: Belyy et al Elife. 2022 Jun 22;11:e74342. doi: 10.7554/eLife.74342.

Backbone

• Vector backbone: pFlexible
• Vector type: CRISPR

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: ERN1 homology arms
• Alt name: IRE1alpha
• Alt name: IRE1a
• Species: H. sapiens (human), Synthetic
• Entrez Gene: ERN1 (a.k.a. IRE1, IRE1P, IRE1a, hIRE1p)
• Tag / Fusion Protein:
  • HaloTag (C terminal on insert)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: unknown (unknown if destroyed)

Resource Information

• Supplemental Documents:
  • pPW3755.gb

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
  • HaloTag Vector Limited Use Label License
• Industry Terms:
  • Not Available to Industry