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Addgene

pCMV-eNme2-T.1-ABE8e
(Plasmid #185668)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 185668 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pCMV with a BR322 origin
  • Vector type
    Mammalian Expression, CRISPR

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    ecTadA(8e)-neNme2-T.2
  • Insert Size (bp)
    3834
  • Mutation
    Nme2Cas9 D16A/E47K/V68M/T123A/D152G/E154K/T396A/H413N/A426S/H452R/E460A/A484T/S629P/N674S/D720A/V765A/H767Y/H771R/V821A/D844A/I859V/W865L/M951R//K1005R/D1028N/S1029A/R1033Y/R1049S/N1064S

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pCMV-eNme2-T.1-ABE8e was a gift from David Liu (Addgene plasmid # 185668 ; http://n2t.net/addgene:185668 ; RRID:Addgene_185668)

  • For your References section:

    High-throughput continuous evolution of compact Cas9 variants targeting single-nucleotide-pyrimidine PAMs. Huang TP, Heins ZJ, Miller SM, Wong BG, Balivada PA, Wang T, Khalil AS, Liu DR. Nat Biotechnol. 2022 Sep 8. pii: 10.1038/s41587-022-01410-2. doi: 10.1038/s41587-022-01410-2. 10.1038/s41587-022-01410-2 PubMed 36076084
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