loSIIN
(Plasmid #185663)

• Purpose: shApc-expressing lentiviruses with varius levels of expression of SIINFEKL
• Depositing Lab: Tyler Jacks
• Publication: Westcott et al Nat Cancer. 2021 Oct;2(10):1071-1085. doi: 10.1038/s43018-021-00247-z. Epub 2021 Sep 30.

Backbone

• Vector backbone: pLenti Trono
• Vector type: Lentiviral

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): NEB Stable
• Copy number: High Copy

Gene/Insert 1

• Gene/Insert name: mScarlet
• Species: Synthetic
• Promoter: Ef1a

Cloning Information for Gene/Insert 1

• Cloning method: Gibson Cloning
• 5′ sequencing primer: N/A
• 3′ sequencing primer: N/A

Gene/Insert 2

• Gene/Insert name: miR-30a
• Promoter: Ef1a

Cloning Information for Gene/Insert 2

• Cloning method: Gibson Cloning
• 5′ sequencing primer: N/A
• 3′ sequencing primer: N/A

Gene/Insert 3

• Gene/Insert name: shApc
• Promoter: Ef1a

Cloning Information for Gene/Insert 3

• Cloning method: Gibson Cloning
• 5′ sequencing primer: N/A
• 3′ sequencing primer: N/A

Gene/Insert 4

• Gene/Insert name: GFP
• Species: Synthetic
• Promoter: Ef1a

Cloning Information for Gene/Insert 4

• Cloning method: Gibson Cloning
• 5′ sequencing primer: N/A
• 3′ sequencing primer: N/A

Resource Information

• A portion of this plasmid was derived from a plasmid made by: Luke Dow mir30 shAPC.

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry