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Addgene

pCW57.1-FRB-MCU-Flag-TetOFF
(Plasmid #185659)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 185659 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pCW57.1-TetOFF
  • Backbone size w/o insert (bp) 7479
  • Total vector size (bp) 8517
  • Vector type
    Mammalian Expression, Lentiviral
  • Selectable markers
    Blasticidin

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    NEB Stable
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    Mitochondrial calcium uniporter
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    1038
  • Entrez Gene
    MCU (a.k.a. C10orf42, CCDC109A, HsMCU)
  • Promoter pTight TRE
  • Tags / Fusion Proteins
    • Flag (C terminal on insert)
    • FRB (N terminal on insert)

Cloning Information

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pCW57.1-FRB-MCU-Flag-TetOFF was a gift from Dipayan Chaudhuri (Addgene plasmid # 185659 ; http://n2t.net/addgene:185659 ; RRID:Addgene_185659)

  • For your References section:

    Mitochondrial calcium uniporter stabilization preserves energetic homeostasis during Complex I impairment. Balderas E, Eberhardt DR, Lee S, Pleinis JM, Sommakia S, Balynas AM, Yin X, Parker MC, Maguire CT, Cho S, Szulik MW, Bakhtina A, Bia RD, Friederich MW, Locke TM, Van Hove JLK, Drakos SG, Sancak Y, Tristani-Firouzi M, Franklin S, Rodan AR, Chaudhuri D. Nat Commun. 2022 May 19;13(1):2769. doi: 10.1038/s41467-022-30236-4. 10.1038/s41467-022-30236-4 PubMed 35589699
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