pEGFP-NDUFV2-mVenus
(Plasmid #185653)

• Purpose: NDUFV2 Complex I sub-unit with C-terminus mVenus tag
• Depositing Lab: Dipayan Chaudhuri
• Publication: Balderas et al Nat Commun. 2022 May 19;13(1):2769. doi: 10.1038/s41467-022-30236-4.

Backbone

• Vector backbone: pEGFP-C1
• Backbone size w/o insert (bp): 3950
• Total vector size (bp): 5450
• Vector type: Mammalian Expression
• Selectable markers: Neomycin (select with G418)

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): NEB Stable
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: NDUFV2
• Species: H. sapiens (human)
• Insert Size (bp): 1500
• Entrez Gene: NDUFV2 (a.k.a. CI-24k, MC1DN7)
• Promoter: CMV
• Tag / Fusion Protein:
  • mVenus (C terminal on insert)

Cloning Information

• Cloning method: Gibson Cloning
• 5′ sequencing primer: CMV-F

Resource Information

• Supplemental Documents:
  • pEGFP-NDUFV2-mVenus.gb

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry