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pGEX-PML B-box1 F158E
(Plasmid #185642)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 185642 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pGEX-6P-3
  • Backbone size w/o insert (bp) 4983
  • Total vector size (bp) 5139
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    promyelocytic leukemia protein
  • Alt name
    TRIM19
  • Alt name
    MYL
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    156
  • Mutation
    F158E
  • Entrez Gene
    PML (a.k.a. MYL, PP8675, RNF71, TRIM19)
  • Tag / Fusion Protein
    • GST (N terminal on backbone)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site BamHI (unknown if destroyed)
  • 3′ cloning site XhoI (unknown if destroyed)
  • 5′ sequencing primer pGEX Fwd
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

codon optimized for expression in E. coli

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pGEX-PML B-box1 F158E was a gift from Irina Bezsonova (Addgene plasmid # 185642 ; http://n2t.net/addgene:185642 ; RRID:Addgene_185642)

  • For your References section:

    Conformational exchange at a C(2)H(2) zinc-binding site facilitates redox sensing by the PML protein. Bregnard TA, Fairchild D, Erlandsen H, Semenova IV, Szczepaniak R, Ahmed A, Weller SK, Korzhnev DM, Bezsonova I. Structure. 2023 Sep 7;31(9):1086-1099.e6. doi: 10.1016/j.str.2023.06.014. Epub 2023 Jul 19. 10.1016/j.str.2023.06.014 PubMed 37473756
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