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Addgene

pLKO-mito-tagRFP
(Plasmid #185599)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 185599 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pLKO-MCS
  • Vector type
    Mammalian Expression, Lentiviral

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    NEB Stable
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    mito-tagRFP-T
  • Alt name
    tagRFP-T targeted to mitochondria
  • Species
    Synthetic

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site unknown (unknown if destroyed)

Resource Information

Terms and Licenses

Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pLKO-mito-tagRFP was a gift from Jarrod Johnson (Addgene plasmid # 185599 ; http://n2t.net/addgene:185599 ; RRID:Addgene_185599)

  • For your References section:

    Genetic Modification of Primary Human Myeloid Cells to Study Cell Migration, Activation, and Organelle Dynamics. Greiner D, Scott TM, Olson GS, Aderem A, Roh-Johnson M, Johnson JS. Curr Protoc. 2022 Aug;2(8):e514. doi: 10.1002/cpz1.514. 10.1002/cpz1.514 PubMed 36018279
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