pLKO-mito-mEm
(Plasmid
#185596)
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PurposeLentiviral construct for expression of mEmerald targeted to mitochondria
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Depositing Lab
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Sequence Information
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 185596 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backbonepLKO-MCS
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Vector typeMammalian Expression, Lentiviral
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)NEB Stable
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Copy numberUnknown
Gene/Insert
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Gene/Insert namemito-mEm
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Alt namemEmerald targeted to mitochondria
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SpeciesSynthetic
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site unknown (unknown if destroyed)
Resource Information
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Supplemental Documents
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Article Citing this Plasmid
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pLKO-mito-mEm was a gift from Jarrod Johnson (Addgene plasmid # 185596 ; http://n2t.net/addgene:185596 ; RRID:Addgene_185596) -
For your References section:
Genetic Modification of Primary Human Myeloid Cells to Study Cell Migration, Activation, and Organelle Dynamics. Greiner D, Scott TM, Olson GS, Aderem A, Roh-Johnson M, Johnson JS. Curr Protoc. 2022 Aug;2(8):e514. doi: 10.1002/cpz1.514. 10.1002/cpz1.514 PubMed 36018279
Map uploaded by the depositor.
