SpVasa-E441Q-GFP
(Plasmid
#185590)
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Purposeto see protein dynamics
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Depositing Lab
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Sequence Information
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 185590 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backbonePSP64
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Vector typeIn vitro transcription (mRNA synthesis)
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameVasa-E441Q
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SpeciesS. purpuratus
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MutationE441Q
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Tag
/ Fusion Protein
- GFP (C terminal on insert)
Cloning Information
- Cloning method Gibson Cloning
Resource Information
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Supplemental Documents
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
Please note: Plasmid contains a L42R mutation in EGFP. It is not known how this mutation affects plasmid function, but the plasmid was functional in the depositor's experiments.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
SpVasa-E441Q-GFP was a gift from Mamiko Yajima (Addgene plasmid # 185590 ; http://n2t.net/addgene:185590 ; RRID:Addgene_185590) -
For your References section:
A tumor suppressor Retinoblastoma1 is essential for embryonic development in the sea urchin. Fernandez-Nicolas A, Xu D, Yajima M. Dev Dyn. 2019 Dec;248(12):1273-1285. doi: 10.1002/dvdy.113. Epub 2019 Oct 1. 10.1002/dvdy.113 PubMed 31515896
