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Addgene

membrane-mCherry-SpVasa-C3
(Plasmid #185587)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 185587 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    PSP64
  • Vector type
    In vitro transcription (mRNA synthesis)

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    Vasa-C3
  • Species
    S. purpuratus
  • Mutation
    C3 mutant
  • Tags / Fusion Proteins
    • membrane targeting sequence from Neuromodulin (MLCCMRRTKQVEKNDDDQKI) (N terminal on insert)
    • mCherry (N terminal on insert)

Cloning Information

  • Cloning method Gibson Cloning

Terms and Licenses

Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    membrane-mCherry-SpVasa-C3 was a gift from Mamiko Yajima (Addgene plasmid # 185587 ; http://n2t.net/addgene:185587 ; RRID:Addgene_185587)

  • For your References section:

    A tumor suppressor Retinoblastoma1 is essential for embryonic development in the sea urchin. Fernandez-Nicolas A, Xu D, Yajima M. Dev Dyn. 2019 Dec;248(12):1273-1285. doi: 10.1002/dvdy.113. Epub 2019 Oct 1. 10.1002/dvdy.113 PubMed 31515896
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