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Addgene

membrane-HsPLK1-dead
(Plasmid #185572)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 185572 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    PSP64
  • Vector type
    In vitro transcription (mRNA synthesis)

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    PLK1-dead
  • Species
    H. sapiens (human)
  • Mutation
    K82R kinase-dead mutant
  • Entrez Gene
    PLK1 (a.k.a. PLK, STPK13)
  • Tag / Fusion Protein
    • membrane targeting sequence from Neuromodulin (MLCCMRRTKQVEKNDDDQKI) (N terminal on insert)

Cloning Information

  • Cloning method Gibson Cloning

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    membrane-HsPLK1-dead was a gift from Mamiko Yajima (Addgene plasmid # 185572 ; http://n2t.net/addgene:185572 ; RRID:Addgene_185572)

  • For your References section:

    Evolutionary modification of AGS protein contributes to formation of micromeres in sea urchins. Poon J, Fries A, Wessel GM, Yajima M. Nat Commun. 2019 Aug 22;10(1):3779. doi: 10.1038/s41467-019-11560-8. 10.1038/s41467-019-11560-8 PubMed 31439829
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