membrane-HsPLK1
(Plasmid
#185571)
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Purposeto see protein dynamics
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 185571 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonePSP64
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Vector typeIn vitro transcription (mRNA synthesis)
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert namePLK1
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SpeciesH. sapiens (human)
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Entrez GenePLK1 (a.k.a. PLK, STPK13)
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Tag
/ Fusion Protein
- membrane targeting sequence from Neuromodulin (MLCCMRRTKQVEKNDDDQKI) (N terminal on insert)
Cloning Information
- Cloning method Gibson Cloning
Resource Information
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Supplemental Documents
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
membrane-HsPLK1 was a gift from Mamiko Yajima (Addgene plasmid # 185571 ; http://n2t.net/addgene:185571 ; RRID:Addgene_185571) -
For your References section:
Evolutionary modification of AGS protein contributes to formation of micromeres in sea urchins. Poon J, Fries A, Wessel GM, Yajima M. Nat Commun. 2019 Aug 22;10(1):3779. doi: 10.1038/s41467-019-11560-8. 10.1038/s41467-019-11560-8 PubMed 31439829