AAV-CMV-Cyp26a1
(Plasmid #185563)

• Purpose: AAV construct to ubiquitously express mouse Cyp26a1
• Depositing Lab: Connie Cepko
• Publication: Amamoto et al Elife. 2022 Mar 22;11. pii: 76389. doi: 10.7554/eLife.76389.

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Backbone

• Vector backbone: AAV
• Vector type: AAV

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): NEB Stable
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: Cyp26a1
• Species: M. musculus (mouse)
• Entrez Gene: Cyp26a1 (a.k.a. Cyp26, P450RA, P450RAI, RAH)
• Promoter: CMV

Cloning Information

• Cloning method: Gibson Cloning
• 5′ sequencing primer: aagaattccgcgggcccgggatccaATGGGGCTCCCGGC
• 3′ sequencing primer: gccctcgaggtcgacggtatcgataTCAGATATCTCCCTGGAAGTGGGT

Resource Information

• Supplemental Documents:
  • AAV-CMV-Cyp26a1.gb

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry