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Addgene

pLV-GG-hUbC-EBPF2-gRNA-RBMS3
(Plasmid #185557)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 185557 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pLV
  • Backbone size w/o insert (bp) 10451
  • Total vector size (bp) 11493
  • Vector type
    Mammalian Expression, Lentiviral, CRISPR
  • Selectable markers
    EBFP2

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    SURE2
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    RBMS3 gRNAs
  • gRNA/shRNA sequence
    CCCCGAACACCATGTCACTC; AGCAGCAACTAAGCTGTACA; GAAGCTGATCTGCAAGGATT; TTACGAACGCTGGCAATTGA
  • Species
    H. sapiens (human)

Cloning Information

  • Cloning method Unknown
  • 5′ sequencing primer CAGCACAAAAGGAAACTCACC; GAGGGCCTATTTCCCATGATT; TCGCTATGTGTTCTGGGAAA; cctgctgaagctctagtac
    • (Common Sequencing Primers)

Resource Information

  • Supplemental Documents
  • A portion of this plasmid was derived from a plasmid made by
    This plasmid was adapted from pLV GG hUbC-dsRED from Charles Gersbach lab. We modified the plasmid to express an EBFP2 reporter (pLV GG hUbC-EBFP2). We then cloned in RBMS3-targeting gRNA

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pLV-GG-hUbC-EBPF2-gRNA-RBMS3 was a gift from Andrew Wilson (Addgene plasmid # 185557 ; http://n2t.net/addgene:185557 ; RRID:Addgene_185557)

  • For your References section:

    CRISPR interference interrogation of COPD GWAS genes reveals the functional significance of desmoplakin in iPSC-derived alveolar epithelial cells. Werder RB, Liu T, Abo KM, Lindstrom-Vautrin J, Villacorta-Martin C, Huang J, Hinds A, Boyer N, Bullitt E, Liesa M, Silverman EK, Kotton DN, Cho MH, Zhou X, Wilson AA. Sci Adv. 2022 Jul 15;8(28):eabo6566. doi: 10.1126/sciadv.abo6566. Epub 2022 Jul 13. 10.1126/sciadv.abo6566 PubMed 35857525
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