pLV-GG-hUbC-EBPF2-gRNA-SOX4
(Plasmid #185552)

• Purpose: Lentiviral backbone with EBFP2 reporter and gRNA targeting SOX4
• Depositing Lab: Andrew Wilson
• Publication: Werder et al Sci Adv. 2022 Jul 15;8(28):eabo6566. doi: 10.1126/sciadv.abo6566. Epub 2022 Jul 13.

Backbone

• Vector backbone: pLV
• Backbone size w/o insert (bp): 10451
• Total vector size (bp): 11493
• Vector type: Mammalian Expression, Lentiviral, CRISPR
• Selectable markers: EBFP2

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): NEB Stable
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: SOX4 gRNAs
• gRNA/shRNA sequence: AAGAGGCCTGTTTCGCTGTC; GTTTCGCTGTCGGGTCTCTA; ACTCCTTAGTGCCGATTCCG; GAGCTACCGAGAGCGCTCGT
• Species: H. sapiens (human)

Cloning Information

• Cloning method: Unknown
• 5′ sequencing primer: CAGCACAAAAGGAAACTCACC; GAGGGCCTATTTCCCATGATT; TCGCTATGTGTTCTGGGAAA; cctgctgaagctctagtac

Resource Information

• Supplemental Documents:
  • pLV-GG-hUbC-EBFP2-gRNA-SOX4.gb
• A portion of this plasmid was derived from a plasmid made by: This plasmid was adapted from pLV GG hUbC-dsRED from Charles Gersbach lab. We modified the plasmid to express an EBFP2 reporter (pLV GG hUbC-EBFP2). We then cloned in SOX4-targeting gRNA

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry