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CamKII-LGI1-pHluorin
(Plasmid #185537)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 185537 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    CamKII
  • Total vector size (bp) 11669
  • Vector type
    Mammalian Expression, Lentiviral

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    NEB Stable
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    LGI1
  • Alt name
    Leucine-rich glioma-inactivated protein 1
  • Species
    R. norvegicus (rat)
  • Entrez Gene
    Lgi1
  • Promoter CamKII
  • Tag / Fusion Protein
    • pHluorin (C terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site BamHI (not destroyed)
  • 3′ cloning site EcoRI (not destroyed)
  • 5′ sequencing primer agtattgtgtatataaggc
    • (Common Sequencing Primers)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    CamKII-LGI1-pHluorin was a gift from Jaime de Juan-Sanz (Addgene plasmid # 185537 ; http://n2t.net/addgene:185537 ; RRID:Addgene_185537)

  • For your References section:

    Activity-driven synaptic translocation of LGI1 controls excitatory neurotransmission. Cuhadar U, Calzado-Reyes L, Pascual-Caro C, Aberra AS, Ritzau-Jost A, Aggarwal A, Ibata K, Podgorski K, Yuzaki M, Geis C, Hallerman S, Hoppa MB, de Juan-Sanz J. Cell Rep. 2024 May 28;43(5):114186. doi: 10.1016/j.celrep.2024.114186. Epub 2024 May 2. 10.1016/j.celrep.2024.114186 PubMed 38700985
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