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Addgene

pGEM_Δflv3.sm
(Plasmid #185533)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 185533 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pGEM®-T easy
  • Backbone manufacturer
    Promega
  • Backbone size w/o insert (bp) 2986
  • Total vector size (bp) 5543
  • Modifications to backbone
    digested with XhoI and SalI and recircularized
  • Vector type
    Synthetic Biology

Growth in Bacteria

  • Bacterial Resistance(s)
    Spectinomycin and Streptomycin, 50 & 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    aadA gene flanked by flv3 upstream and downstream regions
  • Insert Size (bp)
    2557

Cloning Information

  • Cloning method Ligation Independent Cloning
  • 5′ sequencing primer TGTAAAACGACGGCCAGT
  • 3′ sequencing primer CAGGAAACAGCTATGACC
    • (Common Sequencing Primers)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pGEM_Δflv3.sm was a gift from Paula Tamagnini (Addgene plasmid # 185533 ; http://n2t.net/addgene:185533 ; RRID:Addgene_185533)

  • For your References section:

    Engineering cyanobacterial chassis for improved electron supply toward a heterologous ene-reductase. Spasic J, Oliveira P, Pacheco C, Kourist R, Tamagnini P. J Biotechnol. 2022 Dec 10;360:152-159. doi: 10.1016/j.jbiotec.2022.11.005. Epub 2022 Nov 9. 10.1016/j.jbiotec.2022.11.005 PubMed 36370921
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