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pCS2-foxd4l1.1-QR-myc
(Plasmid #185521)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 185521 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pCS2+
  • Vector type
    In vitro transcription

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    foxd4l1.1
  • Alt name
    foxd5
  • Species
    X. laevis (frog)
  • Insert Size (bp)
    1160
  • Mutation
    replaces Q>R upstream of GARQYLNI
  • Tag / Fusion Protein
    • myc (N terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site unknown (unknown if destroyed)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pCS2-foxd4l1.1-QR-myc was a gift from Sally Moody (Addgene plasmid # 185521 ; http://n2t.net/addgene:185521 ; RRID:Addgene_185521)

  • For your References section:

    Specific domains of FoxD4/5 activate and repress neural transcription factor genes to control the progression of immature neural ectoderm to differentiating neural plate. Neilson KM, Klein SL, Mhaske P, Mood K, Daar IO, Moody SA. Dev Biol. 2012 May 15;365(2):363-75. doi: 10.1016/j.ydbio.2012.03.004. Epub 2012 Mar 10. 10.1016/j.ydbio.2012.03.004 PubMed 22425621
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