pCS2-foxd4l1.1-A6-myc
(Plasmid
#185507)
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PurposeExpression of Xenopus laevis foxd4
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
---|---|---|---|---|---|
Plasmid | 185507 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepCS2+
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Vector typeIn vitro transcription
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert namefoxd4l1.1
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Alt namefoxd5
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SpeciesX. laevis (frog)
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Insert Size (bp)1160
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MutationFSIENIM to AAAAAAM
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Tag
/ Fusion Protein
- myc (N terminal on insert)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site unknown (unknown if destroyed)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pCS2-foxd4l1.1-A6-myc was a gift from Sally Moody (Addgene plasmid # 185507 ; http://n2t.net/addgene:185507 ; RRID:Addgene_185507) -
For your References section:
Specific domains of FoxD4/5 activate and repress neural transcription factor genes to control the progression of immature neural ectoderm to differentiating neural plate. Neilson KM, Klein SL, Mhaske P, Mood K, Daar IO, Moody SA. Dev Biol. 2012 May 15;365(2):363-75. doi: 10.1016/j.ydbio.2012.03.004. Epub 2012 Mar 10. 10.1016/j.ydbio.2012.03.004 PubMed 22425621