pCS2-foxd4l1.1-EnR
(Plasmid
#185497)
-
PurposeExpression of Xenopus laevis foxd4
-
Depositing Lab
-
Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
---|---|---|---|---|---|
Plasmid | 185497 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
-
Vector backbonepCS2+
-
Vector typeIn vitro transcription
Growth in Bacteria
-
Bacterial Resistance(s)Ampicillin, 100 μg/mL
-
Growth Temperature37°C
-
Growth Strain(s)DH5alpha
-
Copy numberHigh Copy
Gene/Insert
-
Gene/Insert namefoxd4l1.1
-
Alt namefoxd5
-
SpeciesX. laevis (frog)
-
Insert Size (bp)1480
-
Mutationfusion of winged helix domain with EnR domain
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site unknown (unknown if destroyed)
- 3′ cloning site unknown (unknown if destroyed)
- 5′ sequencing primer NA (Common Sequencing Primers)
Terms and Licenses
-
Academic/Nonprofit Terms
-
Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
-
For your Materials & Methods section:
pCS2-foxd4l1.1-EnR was a gift from Sally Moody (Addgene plasmid # 185497 ; http://n2t.net/addgene:185497 ; RRID:Addgene_185497) -
For your References section:
foxD5 plays a critical upstream role in regulating neural ectodermal fate and the onset of neural differentiation. Yan B, Neilson KM, Moody SA. Dev Biol. 2009 May 1;329(1):80-95. doi: 10.1016/j.ydbio.2009.02.019. Epub 2009 Feb 26. 10.1016/j.ydbio.2009.02.019 PubMed 19250931