MAC3-tag-N
(Plasmid
#185482)
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Purpose(Empty Backbone) Gateway compatible destination vectors containing StrepIII/HA/UltralD
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
---|---|---|---|---|---|
Plasmid | 185482 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepcDNA5/FRT/TO
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Vector typeMammalian Expression, Bacterial Expression
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Selectable markersHygromycin
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Tag
/ Fusion Protein
- StrepIII-HA-UltraID (N terminal on backbone)
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)ccdB Survival
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Copy numberHigh Copy
Cloning Information
- Cloning method Restriction Enzyme
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
MAC3-tag-N was a gift from Markku Varjosalo (Addgene plasmid # 185482 ; http://n2t.net/addgene:185482 ; RRID:Addgene_185482) -
For your References section:
Physical and functional interactome atlas of human receptor tyrosine kinases. Salokas K, Liu X, Ohman T, Chowdhury I, Gawriyski L, Keskitalo S, Varjosalo M. EMBO Rep. 2022 Apr 5:e54041. doi: 10.15252/embr.202154041. 10.15252/embr.202154041 PubMed 35384245