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Addgene

MAC3-tag-N
(Plasmid #185482)

Ordering

This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 185482 Standard format: Plasmid sent in bacteria as agar stab 1 $85

Backbone

  • Vector backbone
    pcDNA5/FRT/TO
  • Vector type
    Mammalian Expression, Bacterial Expression
  • Selectable markers
    Hygromycin
  • Tag / Fusion Protein
    • StrepIII-HA-UltraID (N terminal on backbone)

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    ccdB Survival
  • Copy number
    High Copy

Cloning Information

  • Cloning method Restriction Enzyme

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    MAC3-tag-N was a gift from Markku Varjosalo (Addgene plasmid # 185482 ; http://n2t.net/addgene:185482 ; RRID:Addgene_185482)
  • For your References section:

    Physical and functional interactome atlas of human receptor tyrosine kinases. Salokas K, Liu X, Ohman T, Chowdhury I, Gawriyski L, Keskitalo S, Varjosalo M. EMBO Rep. 2022 Apr 5:e54041. doi: 10.15252/embr.202154041. 10.15252/embr.202154041 PubMed 35384245