pSEVA251_ Ptrc.x.tetO2::D-HicDH
(Plasmid
#185456)
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PurposeCDS of D-HicDH codon optimized for expression in Synechocystis sp. PCC 6803 under the control of the synthetic promoter Ptrc.x.tetO2 and the RBS BBa_B0030 in pSEVA251 plasmid.
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Depositing Lab
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Publication
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Sequence Information
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 185456 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backbonepSEVA251
- Backbone size w/o insert (bp) 5438
- Total vector size (bp) 6368
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Vector typeBacterial Expression
Growth in Bacteria
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Bacterial Resistance(s)Kanamycin, 50 μg/mL
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Growth Temperature30°C
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Growth Strain(s)DH5alpha
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Growth instructionssee additional file or original article
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Copy numberLow Copy
Gene/Insert
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Gene/Insert nameD-HicDH from Lactobacillus paracasei DSM 20008
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Alt namehydroxyisocaproate dehydrogenase from Lactobacillus paracasei DSM 20008
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SpeciesLactobacillus paracasei DSM 20008
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Insert Size (bp)1029
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GenBank IDM26929
- Promoter Ptrc.x.tetO2
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site EcoRI (not destroyed)
- 3′ cloning site PstI (not destroyed)
- 5′ sequencing primer AGGGCGGCGGATTTGTCC
- 3′ sequencing primer GCGGCAACCGAGCGTTC (Common Sequencing Primers)
Resource Information
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Supplemental Documents
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pSEVA251_ Ptrc.x.tetO2::D-HicDH was a gift from Wolfgang Kroutil (Addgene plasmid # 185456 ; http://n2t.net/addgene:185456 ; RRID:Addgene_185456) -
For your References section:
Expression and activity of heterologous hydroxyisocaproate dehydrogenases in Synechocystis sp. PCC 6803 ΔhoxYH. Jurkaš V, Winkler CK, Poschenrieder S, Oliveira P, Pacheco CC, Ferreira EA, Weissensteiner F, De Santis P, Kara S, Kourist R, Tamagnini P, Kroutil W. Engineering Microbiology, 2022 10.1016/j.engmic.2021.100008
