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pSEVA251_ Ptrc.x.tetO2::D-HicDH
(Plasmid #185456)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 185456 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pSEVA251
  • Backbone size w/o insert (bp) 5438
  • Total vector size (bp) 6368
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    30°C
  • Growth Strain(s)
    DH5alpha
  • Growth instructions
    see additional file or original article
  • Copy number
    Low Copy

Gene/Insert

  • Gene/Insert name
    D-HicDH from Lactobacillus paracasei DSM 20008
  • Alt name
    hydroxyisocaproate dehydrogenase from Lactobacillus paracasei DSM 20008
  • Species
    Lactobacillus paracasei DSM 20008
  • Insert Size (bp)
    1029
  • GenBank ID
    M26929
  • Promoter Ptrc.x.tetO2

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site EcoRI (not destroyed)
  • 3′ cloning site PstI (not destroyed)
  • 5′ sequencing primer AGGGCGGCGGATTTGTCC
  • 3′ sequencing primer GCGGCAACCGAGCGTTC
    • (Common Sequencing Primers)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pSEVA251_ Ptrc.x.tetO2::D-HicDH was a gift from Wolfgang Kroutil (Addgene plasmid # 185456 ; http://n2t.net/addgene:185456 ; RRID:Addgene_185456)

  • For your References section:

    Expression and activity of heterologous hydroxyisocaproate dehydrogenases in Synechocystis sp. PCC 6803 ΔhoxYH. Jurkaš V, Winkler CK, Poschenrieder S, Oliveira P, Pacheco CC, Ferreira EA, Weissensteiner F, De Santis P, Kara S, Kourist R, Tamagnini P, Kroutil W. Engineering Microbiology, 2022 10.1016/j.engmic.2021.100008
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