pLVX-EF1alpha-nCoV2019-N-meGFP-IRES-Puro
(Plasmid #185451)

• Purpose: Expresses SARS-CoV-2 nucleocapsid protein with a c-terminal monomeric EGFP in mammalian cells
• Depositing Lab: Ahmet Yildiz
• Publication: Jack et al PLoS Biol. 2021 Oct 11;19(10):e3001425. doi: 10.1371/journal.pbio.3001425. eCollection 2021 Oct.

Backbone

• Vector backbone: pLVX
• Backbone size w/o insert (bp): 8948
• Total vector size (bp): 10785
• Vector type: Mammalian Expression, Lentiviral
• Selectable markers: Puromycin

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): NEB Stable
• Copy number: Unknown

Gene/Insert

• Gene/Insert name: Nucleocapsid
• Species: SARS-CoV-2
• Insert Size (bp): 1257
• Entrez Gene: N (a.k.a. GU280_gp10)
• Promoter: EF1alpha
• Tag / Fusion Protein:
  • mEGFP (C terminal on insert)

Cloning Information

• Cloning method: Gibson Cloning
• 5′ sequencing primer: GCCAGCTTGGCACTTGATG
• 3′ sequencing primer: AAT AAC ATA TAG ACA AAC GC

Resource Information

• Supplemental Documents:
  • plvx-ef1alpha-ncov2019-n-megfp-ires-puro (1).gb

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry