Skip to main content
Addgene

pLVX-EF1alpha-nCoV2019-N-YBBR-2xStrep-IRES-Puro
(Plasmid #185450)

Print

Ordering

This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 185450 Standard format: Plasmid sent in bacteria as agar stab 1 $85
Add to Cart

Backbone

  • Vector backbone
    pLVX
  • Backbone size w/o insert (bp) 8948
  • Total vector size (bp) 10206
  • Vector type
    Mammalian Expression, Lentiviral
  • Selectable markers
    Puromycin

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    NEB Stable
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    Nucleocapsid
  • Species
    SARS-CoV-2
  • Entrez Gene
    N (a.k.a. GU280_gp10)
  • Promoter EF1alpha
  • Tags / Fusion Proteins
    • Strep-Tag II (C terminal on insert)
    • YBBR (C terminal on insert)

Cloning Information

  • Cloning method Gibson Cloning
  • 5′ sequencing primer GCCAGCTTGGCACTTGATG
  • 3′ sequencing primer AAT AAC ATA TAG ACA AAC GC
    • (Common Sequencing Primers)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pLVX-EF1alpha-nCoV2019-N-YBBR-2xStrep-IRES-Puro was a gift from Ahmet Yildiz (Addgene plasmid # 185450 ; http://n2t.net/addgene:185450 ; RRID:Addgene_185450)

  • For your References section:

    SARS-CoV-2 nucleocapsid protein forms condensates with viral genomic RNA. Jack A, Ferro LS, Trnka MJ, Wehri E, Nadgir A, Nguyenla X, Fox D, Costa K, Stanley S, Schaletzky J, Yildiz A. PLoS Biol. 2021 Oct 11;19(10):e3001425. doi: 10.1371/journal.pbio.3001425. eCollection 2021 Oct. 10.1371/journal.pbio.3001425 PubMed 34634033
Related items: