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pBig2ab zz TEV YBBR POT1 ZZ TEV TPP1 MBP TEV TIN2 ZZ TEV TRF1 (4comp1)
(Plasmid #185447)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 185447 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pBig2ab
  • Backbone size w/o insert (bp) 7000
  • Total vector size (bp) 17027
  • Vector type
    Insect Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Chloramphenicol and Gentamicin, 25 & 10 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert 1

  • Gene/Insert name
    TRF1
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    1128
  • GenBank ID
    NM_017489.3
  • Entrez Gene
    TERF1 (a.k.a. PIN2, TRBF1, TRF, TRF1, hTRF1-AS, t-TRF1)
  • Tag / Fusion Protein
    • ZZ (N terminal on insert)

Cloning Information for Gene/Insert 1

  • Cloning method Gibson Cloning
  • 5′ sequencing primer ctcggcctcggccaccaggcc
  • 3′ sequencing primer gtgaattatgtgctaagtga
    • (Common Sequencing Primers)

Gene/Insert 2

  • Gene/Insert name
    POT1
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    1907
  • GenBank ID
    NP_056265.2
  • Entrez Gene
    POT1 (a.k.a. CMM10, CRMCC3, GLM9, HPOT1, PFBMFT8, TPDS3)
  • Tags / Fusion Proteins
    • ZZ (N terminal on insert)
    • YBBR (N terminal on insert)

Cloning Information for Gene/Insert 2

  • Cloning method Gibson Cloning
  • 5′ sequencing primer CTTGAATCTTCAGCCTGTGAAAGCGAAC
  • 3′ sequencing primer CACCCCTGAATCAACTTAAGGGTGG
    • (Common Sequencing Primers)

Gene/Insert 3

  • Gene/Insert name
    TPP1
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    1371
  • GenBank ID
    AAX82621.1
  • Entrez Gene
    TPP1 (a.k.a. CLN2, GIG1, LPIC, SCAR7, TPP-1)
  • Tag / Fusion Protein
    • ZZ (N terminal on insert)

Cloning Information for Gene/Insert 3

  • Cloning method Gibson Cloning
  • 5′ sequencing primer CTGGTCATTCTCAGAGATGC
  • 3′ sequencing primer GAAATGCGGGAGGACCAGGA
    • (Common Sequencing Primers)

Gene/Insert 4

  • Gene/Insert name
    TIN2
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    1059
  • GenBank ID
    NM_012461.3
  • Entrez Gene
    TINF2 (a.k.a. DKCA3, TIN2)
  • Tag / Fusion Protein
    • MBP (N terminal on insert)

Cloning Information for Gene/Insert 4

  • Cloning method Gibson Cloning
  • 5′ sequencing primer GCT ACG CCC CTG GTG GCG GG
  • 3′ sequencing primer CTA AAG GGA AAC AGC ATG AC
    • (Common Sequencing Primers)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pBig2ab zz TEV YBBR POT1 ZZ TEV TPP1 MBP TEV TIN2 ZZ TEV TRF1 (4comp1) was a gift from Ahmet Yildiz (Addgene plasmid # 185447 ; http://n2t.net/addgene:185447 ; RRID:Addgene_185447)

  • For your References section:

    Compartmentalization of telomeres through DNA-scaffolded phase separation. Jack A, Kim Y, Strom AR, Lee DSW, Williams B, Schaub JM, Kellogg EH, Finkelstein IJ, Ferro LS, Yildiz A, Brangwynne CP. Dev Cell. 2022 Jan 24;57(2):277-290.e9. doi: 10.1016/j.devcel.2021.12.017. 10.1016/j.devcel.2021.12.017 PubMed 35077681
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