LDB59
(Plasmid
#185436)
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PurposeNUP1 CEN URA3 plasmid
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Depositing Labs
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Publication
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Sequence Information
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 185436 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backbonepRS316
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Vector typeYeast Expression
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Selectable markersURA3
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameNUP1
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SpeciesS. cerevisiae (budding yeast)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site unknown (unknown if destroyed)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
Also used in Harper et al. (2008) "Mutations affecting spindle pole body and mitotic exit network function are synthetically lethal with a deletion of the nucleoporin NUP1 in S. cerevisiae." Current genetics. https://doi.org/10.1007/s00294-007-0168-4. PMID: 18058101
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
LDB59 was a gift from Ken Belanger & Laura Davis (Addgene plasmid # 185436 ; http://n2t.net/addgene:185436 ; RRID:Addgene_185436) -
For your References section:
nup1 mutants exhibit pleiotropic defects in nuclear pore complex function. Bogerd AM, Hoffman JA, Amberg DC, Fink GR, Davis LI. J Cell Biol. 1994 Oct . 127(2):319-32. 10.1083/jcb.127.2.319 PubMed 7929578
