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Addgene

LDB34
(Plasmid #185431)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 185431 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pRS314
  • Vector type
    Yeast Expression
  • Selectable markers
    TRP1

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    NUP1
  • Species
    S. cerevisiae (budding yeast)
  • Mutation
    Truncation at amino acid 1041 from the C-term of Nup1

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site unknown (unknown if destroyed)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Please note: Plasmid contains an additional 22 amino acids (LKDIYSYIPSTYTLHMKLLEKF) on the C-terminus of Nup1 compared to the NCBI reference sequence NP_014741.1. It is not known how this affect the plasmid's function.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    LDB34 was a gift from Ken Belanger & Laura Davis (Addgene plasmid # 185431 ; http://n2t.net/addgene:185431 ; RRID:Addgene_185431)

  • For your References section:

    nup1 mutants exhibit pleiotropic defects in nuclear pore complex function. Bogerd AM, Hoffman JA, Amberg DC, Fink GR, Davis LI. J Cell Biol. 1994 Oct . 127(2):319-32. 10.1083/jcb.127.2.319 PubMed 7929578
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