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Addgene

LDB203
(Plasmid #185424)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 185424 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pGEX-2TK
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    NUP1
  • Species
    S. cerevisiae (budding yeast)
  • Mutation
    Nup1 N-terminal domain only
  • Tag / Fusion Protein
    • GST (N terminal on backbone)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site unknown (unknown if destroyed)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Please note: The plasmid contains S101G and T354A mutations in Nup1 compared to the NCBI reference sequence NP_014741.1. It is not known how these mutation affect plasmid function.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    LDB203 was a gift from Ken Belanger & Laura Davis (Addgene plasmid # 185424 ; http://n2t.net/addgene:185424 ; RRID:Addgene_185424)

  • For your References section:

    Using affinity chromatography to investigate novel protein-protein interactions in an undergraduate cell and molecular biology lab course. Belanger KD. CBE Life Sci Educ. 2009 Fall;8(3):214-25. doi: 10.1187/cbe.09-03-0019. 10.1187/cbe.09-03-0019 PubMed 19723816
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