LDB107
(Plasmid #185423)

• Purpose: NUP1-HA CEN TRP1 AmpR. Yeast shuttle plasmid for expression of hemagluttinin tagged NUP1 nucleoporin
• Depositing Labs: Ken Belanger, Laura Davis
• Publication: Belanger et al J Cell Biol. 1994 Aug;126(3):619-30. doi: 10.1083/jcb.126.3.619.

Backbone

• Vector backbone: pRS314
• Vector type: Yeast Expression
• Selectable markers: TRP1

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: NUP1
• Species: S. cerevisiae (budding yeast)
• Tag / Fusion Protein:
  • HA

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: unknown (unknown if destroyed)
• 3′ cloning site: unknown (unknown if destroyed)
• 5′ sequencing primer: NA

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

Also used in Finn et al. (2013) "A subset of FG-nucleoporins is necessary for efficient Msn5-mediated nuclear protein export." Biochim Biophys Acta. doi.org/10.1016/j.bbamcr.2012.12.020. PMID: 23295456

Please note: HA-tag is in the middle of NUP1and the ORF in reverse orientation (downstream of T3 promoter) compared to other similar plasmids