Skip to main content
Addgene

KBB11
(Plasmid #185422)

Print

Ordering

This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 185422 Standard format: Plasmid sent in bacteria as agar stab 1 $85
Add to Cart

Backbone

  • Vector backbone
    pRS314
  • Vector type
    Yeast Expression
  • Selectable markers
    TRP1

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    KAP60
  • Species
    S. cerevisiae (budding yeast)
  • Promoter endogenous

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site unknown (unknown if destroyed)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Please note: KAP60/SRP1 is in the opposite orientation of other plasmids in the deposit making it downstream of the T3 promoter as opposed to downstream of T7 promoter.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    KBB11 was a gift from Ken Belanger & Laura Davis (Addgene plasmid # 185422 ; http://n2t.net/addgene:185422 ; RRID:Addgene_185422)

  • For your References section:

    Genetic and physical interactions between Srp1p and nuclear pore complex proteins Nup1p and Nup2p. Belanger KD, Kenna MA, Wei S, Davis LI. J Cell Biol. 1994 Aug;126(3):619-30. doi: 10.1083/jcb.126.3.619. 10.1083/jcb.126.3.619 PubMed 8045927
Related items: