pCS2+ Zf cdh1-deltaCyot-GFP
(Plasmid #185412)

• Purpose: GFP tagged zebrafish cdh1 lacking the cytoplasmic domain.
• Depositing Lab: Nicolas David
• Publication: Boutillon et al Dev Cell. 2022 May 17. pii: S1534-5807(22)00329-X. doi: 10.1016/j.devcel.2022.05.001.

Backbone

• Vector backbone: pCS2+
• Backbone size w/o insert (bp): 4095
• Total vector size (bp): 6996
• Vector type: Mammalian Expression ; In vitro synthesis of mRNA

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: cdh1
• Alt name: E-cadherin
• Species: D. rerio (zebrafish)
• Insert Size (bp): 2904
• Mutation: Cytoplasmic domain deleted
• GenBank ID: AF364811
• Entrez Gene: cdh1 (a.k.a. E-cadherin)
• Tag / Fusion Protein:
  • eGFP (C terminal on backbone)

Cloning Information

• Cloning method: Gibson Cloning
• 5′ sequencing primer: SP6
• 3′ sequencing primer: T7

Resource Information

• A portion of this plasmid was derived from a plasmid made by: Dr E. Raz, University of Münster

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

Please visit https://www.biorxiv.org/content/10.1101/2021.04.26.441407v2 for bioRxiv preprint.