pCS2+ Lifeact-mCherry
(Plasmid #185408)

• Purpose: Red fluorescent reporter for polymerized actin. For in vitro transcription (SP6) or expression through CMV
• Depositing Lab: Nicolas David
• Publication: Boutillon et al Dev Cell. 2022 May 17. pii: S1534-5807(22)00329-X. doi: 10.1016/j.devcel.2022.05.001.

Backbone

• Vector backbone: pCS2+
• Backbone size w/o insert (bp): 4095
• Total vector size (bp): 4884
• Vector type: Mammalian Expression ; In vitro synthesis of mRNA

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: lifeact
• Insert Size (bp): 795
• Tag / Fusion Protein:
  • mCherry (C terminal on backbone)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: BamHI (not destroyed)
• 3′ cloning site: BamHI (not destroyed)
• 5′ sequencing primer: SP6
• 3′ sequencing primer: M13-rev

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
  • Takara Bio Limited Use Label License (formerly Clontech)
• Industry Terms:
  • Not Available to Industry

Depositor Comments

Please visit https://www.biorxiv.org/content/10.1101/2021.04.26.441407v2 for bioRxiv preprint.